Kotková Lucie
Doctoral training
Genetic and Epigenetic Biomarkers in cancer
Specialization: Pediatrics
Supervisor: Drábek Jiří Ph.D.
Status: Ongoing from 2017.
About me
Researcher and Ph.D. candidate
My undergraduate studies were focused on cancer genetics, with a bachelor's thesis "Molecular and genetic changes in glial tumours" and a master's thesis "Optimization of library technology for multi-parallel sequencing in the colorectal cancer diagnostics".
Now, my main research lies in the field of forensic epigenomics, especially DNA phenotyping and visual characteristic predictions.
I work on developing a model capable of predicting the age of an unknown person based on their DNA extracted from blood or semen samples. My main laboratory tools are bisulfite conversion and its alternatives, qPCR and multiparallel sequencing.
Journals
Development and extensive analytical validation of deep amplicon sequencing for detecting KRAS and NRAS mutations in metastatic colorectal cancer samples. Neoplasma. 2021, 69(1), 203-2015, ISSN: 0028-2685, PMID: 34881628,
Age-related changes in sperm DNA methylation and their forensic and clinical implications. Epigenomics. 2023, ISSN: 1750-1911,
Books & book chapters
Determination of length of nucleic acid fragments by digital capillary electrophoresis, 1.vyd., Olomouc, Palacky University, 2021, 50, 399-408, Dedication: LO1304, ISBN: 978-80-244-6049-9,