Koberna Karel CSc.

User picture
Academic title: RNDr.
Phone: +420 585632184
Groups: IMTM, LEM, STAFF

About me

EDUCATION

1989 – 1994     Title CSc. (equivalent of PhD), Institute of Experimental Medicine AS CR

1983 – 1988     Title MSc. and RNDr. (Rerum naturalium doctor), Faculty of Science, Charles University in Prague; Specialization – Molecular Biology

Area of interest, the basic used technologies and special knowledge

- The effect of the precursors of nucleic acids on their metabolism and cellular metabolism, the development of the new methods for the detection of proliferation activity of mammalian cells and systems for the fast detection of proteins based on the magnetic separations, organisation of chromatin, DNA replication and repair, the development of the new methods for measurement of enzyme activity, study of base excision repair and its use in cancer treatment.

- Immunocytochemical detections, confocal and light microscopy, molecular biology methods, cell cultivation, electron microscopy including electron tomography, high pressure freezing and freeze substitution.

- Other knowledge: work with Microsoft Office, Adobe Photoshop, Corel Draw, ImageJ, Cell Profiler, ilastik, 3D model programs: Rhinoceros, programming in Python.

PUBLICATIONS

  1. Ligasová A., et al. (2023) Int J Mol Sci 24(4): 3674
  2. Hodoň J., et al. (2022) Eur J Med Chem 243: 114777
  3. Ligasová A., et al. (2021) Open Biol 11(10): 210136
  4. Ligasová A., Koberna K. (2021) Int J Mol Sci 22(19): 10759
  5. Ligasová A., Koberna K. (2021) Molecules 26(18): 5515
  6. Ligasová A., Koberna K. (2021) Int J Mol Sci 22(13): 7098
  7. Ligasová A., Koberna K. (2021) Chapter 17, pp 111-120, In Laboratory Techniques in Cellular and Molecular Medicine, Agrawal, K., Bouchal, J., Das, V., Drábek, J., Džubák, P., Hajdúch, M., Koberna, K., Ligasová, A., Mistrík, M., de Sanctis, J.B., et al., Eds. Palacký University Olomouc: Olomouc.
  8. Ligasová A., Koberna K. (2021) Chapter 18, pp 121-132, In Laboratory Techniques in Cellular and Molecular Medicine, Agrawal, K., Bouchal, J., Das, V., Drábek, J., Džubák, P., Hajdúch, M., Koberna, K., Ligasová, A., Mistrík, M., de Sanctis, J.B., et al., Eds. Palacký University Olomouc: Olomouc.
  9. Ligasová A., et al. (2019) Cells, 8(12): E1510
  10. Ligasová A., Koberna K. (2019) Sci. Rep., 9(1): 8701
  11. Ligasová A., Koberna K. (2018) Molecules, 23(11): 3007
  12. Ligasová, A., et al. (2017) PLoS One 12(4): e0175880
  13. Ligasová, A., et al. (2017) PLoS One 12(3): e0174893
  14. Ligasová, A., et al. (2016) Open Biol, 6:150172
  15. Ligasová, A., Koberna, K. (2015) Methods Mol. Biol., 1351:81-92, M. McKenzie ed.
  16. Ligasová, A., et al. (2015) PLoS One 10(7): e0132393
  17. Ligasová, A., et al. (2015) PLoS One, 10(2): e0117459
  18. Cmarko, D., et al. (2014) Methods Mol. Biol., 1117:343-66, John Kuo ed.
  19. Ligasová, A., et al. (2013) PLoS One, 8(6):e66864
  20. Ligasová, A., et al. (2012) PLoS One, 7(12):e52584
  21. Liboska, R., et al. (2012) PLoS One, 7(12):e51679
  22. Ligasová, A., et al. (2011) Parasit. Vectors, 4:162
  23. Ligasová, A., Koberna, K. (2011) DNA replication-Current Advances, Intech, H. Seligmann ed.
  24. Ligasová, A., Koberna K. (2010) Nucleic Acids Res., 38(16):e167
  25. Ligasová, A., et al. (2009) J. Struct. Biol., 165(3):204-213
  26. Cvačková, Z., et al. (2008) Chromosoma, 117(5):487-497
  27. Kalmárová, M., et al. (2007) J. Struct. Biol., 160(1):49-56
  28. Cmarko, D., Koberna, K. (2007) Methods Mol. Biol. 369:213-228, J. Kuo ed.
  29. Stavreva, D. A., et al. (2006) Mol. Cell Biol., 26:5131-5145
  30. Smirnov, E., et al. (2006) Folia Biol., 52:59-70
  31. Fidlerová ,H., et al. (2005) J. Cell Biochem., 94:899-916
  32. Pliss, A., et al. (2005) J. Cell Biochem., 15:554-565
  33. Li, J., et al. (2005) EMBO J., 12:120-127
  34. Koberna, K., et al. (2005) J. Cell Biochem., 94(1):126-138
  35. Večeřová, J., et al. (2004) Mol. Biol. Cell, 15:4904-4910
  36. Raška, I., et al. (2004) Biol. Cell, 96:579-594
  37. Malínský, J., et al. (2002) J. Struct. Biol., 140:227-231
  38. Koberna, K., et al. (2002) J. Cell Biol., 157:743-748
  39. Kopský, V., et al. (2002) Fol. Biol. (Prague), 48:69-72
  40. Staněk, D., et al. (2001) Chromosoma, 110(7):460-470
  41. Malínský, J., et al. (2001) J. Cell Sci., 114(Pt 4):747-750
  42. Koberna, K., et al., (2000) Acta Histochem., 102(1):15-20
  43. Melčák, I., et al. (2000) Mol. Biol. Cell., 11(2):497-510
  44. Koberna, K., et al. (1999) Chromosoma, 108(5):325-335
  45. Koberna, K., et al. (1998) Reprod. Nutr. Dev., 38(1):117-126
  46. Schul, W., et al. (1996) EMBO J., 15(11):2883-2892
  47. Raška, I., et al. (1995) J. Struct. Biol., 114(1):1-22
  48. Raška, I., et al. (1992) Cell Biol. Int. Rep., 16(8):771-789
  49. Raška, I., et al. (1989) Exp. Cell Res., 184(1):81-89
  50. Raška, I., et al. (1987) Histochem. J., 19(10-11):603

PATENTS                                      

  • Czech: 302613; 303230; 303251; 303252; 306187; 307016; 307807; 307304; 307454; 307415; 308519; 308385; 308387, 309218
  • European: 3186387, 3395956, 3558526

UTILITY MODELS

  • Czech: 28964; 34386; 34442

INDUSTRIAL DESIGN

  • Czech: 3668

HONOURS AND AWARDS

2003     Premium of Otto Wichterle for young scientists AS CR

Journals

LIGASOVÁ, A., M. HOREJSOVA, D. FRIEDECKÝ, E. POKORNA, P. KLENER, K. KOBERNA
A hybrid RNA-based reporter assay for robust quantification of cytidine deaminase activity. Nucleic Acids Research. 2026, 54(11), gkag588, ISSN: 0305-1048, PMID: 42273913,  PDF.
LIGASOVÁ, A., B. PISKLÁKOVÁ, D. FRIEDECKÝ, K. KOBERNA
A new technique for the analysis of metabolic pathways of cytidine analogues and cytidine deaminase activities in cells. Scientific Reports. 2023, 13(1), 20530, ISSN: 2045-2322 , PMID: 37993628,
LIGASOVÁ, A., I. FRYDRYCH, K. KOBERNA
Basic Methods of Cell Cycle Analysis. International Journal of Molecular Sciences. 2023, 24(12), 3674, ISSN: 1422-0067, PMID: 36835083,  PDF.

Books & book chapters

LIGASOVÁ, A., K. KOBERNA
In situ detection of overall and replicated mitochondrial DNA in mammalian cells , 1.vyd., Olomouc, Palacky University, 2021, 18, 129-140, Dedication: LO1304, ISBN: 978-80-244-6049-9,
LIGASOVÁ, A., K. KOBERNA
In situ detection of replicated DNA in mammalian cell nuclei , 1.vyd., Olomouc, Palacky University, 2021, 17, 119-128, Dedication: LO1304, ISBN: 978-80-244-6049-9,
DRÁBEK, J., V. DAS, P. DŽUBÁK, J. SROVNAL, J. BOUCHAL, M. HAJDÚCH, K. KOBERNA, A. LIGASOVÁ, M. MISTRÍK, J. DE SANCTIS
Laboratory Techniques in Cellular and Molecular Medicine, 1.vyd., Olomouc, Palacky University, 2021, 436 s, Dedication: LO1304, ISBN: 978-80-244-6049-9,

Intellectual property

A METHOD OF INCREASING THE SIGNAL OF ANTIBODIES RECOGNIZING 5-BROMO-2'-DEOXYURIDINE AND/OR 5-CHLORO-2'-DEOXYURIDINE AND/OR 5-IODO-2'-DEOXYURIDINE AT SITES OF INCORPORATION OF THESE NUCLEOSIDES INTO DNA FOR DNASE I ACTIVITY (Koberna)

Patent: CZ 307807; Granted: 10.4.2019; Ownership: Palacky University, Olomouc; Inventors: Koberna Karel, Ligasová Anna

Status: Terminated

METHOD FOR SELECTIVE REMOVAL OF DNA DYES, KIT FOR SELECTIVE REMOVAL OF DNA DYES AND THEIR USE (Koberna)

1. Patent: CZ 308385; Granted: 4.6.2020; Ownership: Palacky University, Olomouc; Inventors: Koberna Karel, Ligasová Anna

Status: Terminated

KIT FOR MAKING SPECIFIC PYRIMIDINE NUCLEOTIDES IN THE STRUCTURE OF DOUBLE-STANDED DNA TO REACT WITH ANTIBODIES THAT REACT WITH THESE NUCLEOSIDES (Koberna)
  1. Utility Model: CZ 28964, Granted: 14.12.2015, Ownership: Palacky University Olomouc, Inventors: Koberna Karel, Ligasová Anna

Status: Terminated

Master mentorship

Zálešáková Zuzana

Testing and optimization of new non-destructive method for determining the activity of cytidine deaminase for use of various cell lines

Status: Graduated from 2015 to 2017.

Bachelor mentorship

Moštěk Jan

Base excision repairs

Status: Graduated from 2017 to 2021.

Pasecká Martina

Optimization of the method for determining the activity of uracil glycosylase and its application to compare the activity of uracil glycosylase in different cell lines

Kociánová Markéta

Metabolic pathways of deoxycytidine and their impact on the cancer treatment by deoxycytidine analog

Status: Ongoing from 2023.

Open positions

1 Other specializations
Project: Metabolic pathways of deoxycytidine and their impact on the cancer treatment by deoxycytidine analog
Supervisors: Koberna Karel CSc.
Available: 1
Intended for: Bachelor training
Summary: Metabolic pathways of deoxycytidine and their impact on the cancer treatment by deoxycytidine analog

WHERE TO FIND US

Hněvotínská 1333/5
779 00 Olomouc
Czech Republic

+420 585632111
+420 585632180
recepce.umtm@upol.cz
info@imtm.cz

GPS: 49° 35´ 10.1869512" N, 17° 14´ 6.292305" E

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