Available technologies

Institute of molecular and translational medicine, Palacky University Olomouc
  • Genomics laboratory: qPCR for 1536 samples, Affymetrix microarray, Illumina NGS, Sequenom mass spectrometry)
  • Proteomics and metabolomics laboratories: 2x MALDI-TOF / TOF HPLC-MS / MS qTRAP, QTOF, 2x Orbitrap, GC / GC-TOF
  • Microscopy: AFM, Raman microscopy, IR microscopy, confocal microscopy (spinning disc and laser scanning microscopy, super resolution, PALM, SIM, TIRF)
  • High-througput screening / high-content analysis (HTS / HCA) laboratory: chemical compound archiving and automatic processing; three-arm robotic system to test the biological activity of small molecules in BSL2, BSL3 and / or hypoxic environment; detection systems for radioactive , fluorescence and luminescent radiation; broad spectrum and confocal HCA; mass spectrometry screening (RapidFire plus qTRAP)
  • Laboratory for working under hypoxic conditions
  • BSL3 laboratories
  • Department of imaging methods for small animals: optical (fluorescence, luminescence), X-ray, PET / SPECT / CT, ultrasound and MRI methods
  • Laboratory of radiochemical, medicinal and combinatorial chemistry
  • Biobank with more than 15,000 highly characterized samples (tissue, blood, plasma, bone marrow)

 

Central european institute of technology, Masaryk Univesity
  • Flow cytometry and microscopy: BD FACS Aria Fusion, BD FACS Verse, BD Accuri, Olympus ScanR, Zeiss PALM
  • Massive parallel sequencing (“Next Generation Sequencing”): Illumina NextSeq, Illumina MiSeq, Oxford Nanopore MinION
  • Devices for automated preparation of samples and NGS libraries: Fluidigm AccessArray, Wafergen SmartChip, Fluidigm C1, MagCore
  • Microarrays: Affymetrix GeneChip, Agilent SureScan
  • Digital PCR and high-throughput qPCR: Biorad QX200, Life Technologies QuantStudio 12k, Wafergen SmartCycler, Roche 480 LightCycler

 

1st faculty of medicine, Charles University

Complete background for cell culture and protein expression, confocal microscopy, molecular biology equipment, CRISPR technology, real-time PCR with TaqMan® Array Microfluidic Cards (Applied Biosystems), and Agilent analyser of biomolecule (RNA, DNA) integrity.

Available are tools for cultivation of both prokaryotic and eukaryotic cells, including preparation of transfectants, derivation and subsequent propagation of various cell types originating from biopsy material, including primary cultures and cancer stem cells (laminar flow boxes, thermal boxes and incubators with controlled CO2 atmosphere, CellCoulter Z2, equipment for immunomagnetic separation, heated environmental chamber for short-term microscopy observation of cultured cells, small-volume hypoxic incubator, centrifuges, basic inverted microscope, and standard small instruments required for tissue culture).

The laboratories are equipped with spectrofluorimeters for performing fluorimetric analyses in several formats (multiwall plates, cuvettes, etc.), plate as well as cuvette spectrophotometers allowing enzymatic and immunochemical assays such as ELISA. Further, there are tools required for cytochemical and histochemical analyses of biopsy material and cells, including a cryotome. To evaluate and document the results of immunohistochemical analyses, there is a basic epifluorescence and confocal microscope. For routine flow cytometry analyses, the laboratories are equipped with a BD FACS Verse cytometer. In addition, the background and equipment for the portfolio of chromatographic and electrophoretic methods including immunoblot and 2D electrophoresis are available. Since 2018, the Institute of Pathological Physiology of the 1st FM CU has at its disposal the Chemidoc MP laboratory documentation system.

The team has for use the equipment and expertise for stereotactic intracranial implantation of cell suspensions into experimental animals (xenograft model), as well as a transgenic model of mouse glioblastoma and a syngeneic mouse model. The background of the certified animal facility is largely used – with possible use of immunodeficient animals and several relevant mouse knock-out models, allowing verification of the results obtained with the use of in vitro methods in animal models. The Laboratory of Cancer Cell Biology also closely collaborates with the Centre of Advanced Preclinical Imaging Methods (CAPI). This unique department situated in the immediate vicinity of the animal facility has available instruments for imaging methods to be used with the above-mentioned animal models and allows detailed study of cancer biology on these models. One of great advantages is the possibility to combine multiple imaging modalities (e.g., MRI, PET-SPECT-CT, MPI), which is used in the running projects, e.g., for studying the cell distribution of targeted anti-tumour drugs using the PET probes.

 

Institute of microbiology, AS CR, v.v.i.
  • Infrastructure for preparation of genetically engineered microorganisms and purified recombinant antigens
  • Infrastructure for immunization of laboratory animals and laboratory evaluation of immune responses
  • Cytometric and microscopic technologies for phenotypic characterization by immune response and ultrastructural characterization of pathological changes
  • Infrastructure for analyses of molecular binding interactions - SPR technologies
  • Animal facility with unique germ-free and infectious disease animal models for the study of pathophysiology of pertussis, allergies, psoriasis and tumor processes

 

Institute of organic chemistry and biochemistry, AS CR, v.v.i.
  • EPR Spectrometers
  • NMR Spectrometers
  • MS, GC-MS and LC-MS
  • GC Systems
  • HPLC Systems
  • Capillary Electrophoresis
  • Microscale Thermophoresis
  • UV-VIS, IR, Raman and CD
  • Microcalorimetry
  • Optical and Optoelectronic Instruments
  • Cytometers
  • Proteine & Peptide Analysis & Synthesis
  • Radioisotopes
  • X-Ray Diffraction

 

Institute of macromolecular chemistry, AS CR, v.v.i.

•    Vacuum lines for polymer synthesis
•    Technology of radical, ionic and polycondensation polymerizations
•    System for microwave synthesis
•    Equipment for polymer synthesis on application scale
•    Nanospray dryer Buchi B-90 for preparation of nanoparticles
•    Microfluidics technology for preparation of highly defined nano- and microparticles including vesicles
•    Set of instruments for electrochemical deposition of polymers layers 
•    HPLC chromatography including radioactivity detector
•    Gel permeation chromatography including radioactivity detector
•    ALV instrument for static and dynamic light scattering
•    Malvern instrument Mastersizer for small angle static light scattering and particle size distribution
•    Malvern instrument Zetasizer for dynamic and electrophoretic light scattering
•    Zeta potential measurements of flat surfaces
•    FT-IR spectrometer with a holder for studying surfaces and thin layers
•    Spectrofluorometer for steady-state and time-resolved luminescence measurements
•    Confocal laser microscope with FLIM/FCS module
•    Fluorescence plate-reader for high throughput kinetic assays including cell measurements
•    Microcal instrument ITC200 for isothermal titration calorimetry
•    Instrument Bruker E500 Elexys pro electron paramagnetic resonance
•    Scintillation counter MicroBeta
•    Well ionization chamber
•    Spectrometer for gamma radiation
•    Digital 2D autoradiography

 

Nuclear physics institute, AS CR, v.v.i.
  • Supply of medical radionuclides (e.g. 64Cu, 89Zr) and tracers
  • Cyclotron targets of our own design
  • automated technologies for the irradiated targets processing
  • Analytical equipment (gamma-ray spectrometers, ionizing chambers, HPLC-MS with radiodetection etc.)
  • Cell culture laboratory
  • Cyclotrons: isochronous U-120M providing beams of four particles (protons, deuterons, 3He and α particles), and compact TR-24 enabling to bombard targets with proton beam of 24 MeV energy and 300 μA beam current
  • Radiochemical laboratories for the irradiated targets processing, radionuclides separation and analysis, labelled compounds preparation, purification and characterization

 

International center for clinical research, St. Anne’s hospital in Brno
  • NIOBE Stereotaxis Magnetic Navigation System, complete with the Odyssey Cinema System
  • -1.5 T Signa NMR from GE Healthcare, completed with the set-up for anaesthesia
  • Ultrasound systems: VIVID 7 Dimension 4D Breakthrough 2008, VIVID I Breakthrough 2009
  • Cryocath Ablation System
  • Endoscopic and laparoscopic instrumentations
  • ECG TUNNEL and Non Invasive Blood Pressure System (EMKA Technologies)
  • Stellar Telemetry System for Rodents (TSE Systems)
  • Vevo 2100 Imaging System (Visualsonics)
  • Behavioural Set-up: Intellicage System, Phenomaster System, Motorater, Multiconditioning System (TSE Systems)
  • Cryostat (Nikon) and Tissue Processor (Leica TP1020)
  • HPLC with UV
  • MS and CAD detection
  • Microwave Reactor
  • High Pressure Reactor
  • Neuroimaging machines (Biplane angiography system, CT scanner, MRI scanner, Ultrasound machines extracranial and transcranial diagnostcs of arterial disease), X-ray devices with direct digitalizaton
  • NanoAssemblr – preparing the contrast nanopartcles for thrombus and tumour MRI imaging. IT infrastructure for medical databases and research (Cluster consistng of 4 high-performance CPU and GPU computng workstatons -HP Z840, virtualized server farm integrated with the secure hospital IT infrastructure, dedicated PACS app
  • SW StatSof, Inc., 2013. STATISTICA (data analysis sofware system), version 12.
  • SW SAS Insttute Inc., 2011. Base SAS® version 9.4 Procedures Guide. Cary, NC:SAS.
  • SW R Development Core Team (2008).
  • SW REDCap (Research Electronic Data Capture)

 

Institute of experimental medicine, AS CR, v.v.i.
  • Fully equipped laboratories for genomics and proteomics: MiSeq Illumina Sequencer, Microarray Analyzer iScan Illumina, Apogee A-50 Micro Cytometer, QuantStudio ™ 6 Flex Real-Time PCR System, StepOne Plus Real-Time PCR System, Bugbox Plus, Zetasizer Nano ZS.
  • Cell culture laboratories (BSL2)
  • Histological laboratories
  • Patch clamp electrophysiological measurement laboratories
  • Calcium imaging and neurosurgery equipped laboratories (Neurostar stereotactic robot)
  • Wide range of behavioral tests with appropriate analysis (MotoRater, Morris Water Maze, von Frey)
  • Imaging technologies for live and fixed specimens: Zeiss LSM 5 Duo confocal microscope, Olympus FV1200 MPE, two-photon microscope (Ultima IV), electron microscope (Philips Electron Optics), Leica DMI 6000B fluorescence microscope with TissueGnostics image analysis, Lightsheet microscope (LaVision)

 

Institute of Hematology and Blood Transfusion
  • GMP clean room facilities: One workplace for aseptic cell processing in Grade A with a Grade B background (A/B), one manufacturing workplace in Grade A with a Grade C background (A/C), and a closed GMP workplace enabling the expansion of cells up to GMO Class II in an isolator with a Grade C background and closed incubator
  • Cellular manipulation and modification: Miltenyi MACSQuant Tyto GMP cell sorter; ThermoFisher Neon XT electroporation system; GMP-compliant MaxCyte ExPERT GTx electroporation system
  • Genomics facility: ABI 3500 (8-capillary) and ABI 3500xL (24-capillary) sanger sequencing genetic analyzers; 2x Illumina miSeq NGS instruments, GeneMind SURFSeq 5000 high-throughput sequencing platform;10x Genomics Chromium iX high-throughput single-cell RNA sequencing platform; Mission Bio Tapestri single-cell NGS system; Bio-Rad QX200 ddPCR system; real-time cyclers Rotor-Gene Q; high-performance PCR thermal cyclers TAdvanced 96 G - Analytik Jena; automated gel electrophoresis Agilent 4200 TapeStation; automated nucleic acid extractor MagCore Plus II
  • Immune monitoring and flow cytometry facility: Cytek Aurora 5-laser full spectrum flow cytometer; BD FACSymphony A5 SORP 5-laser clasical flow cytometer; BD LSRFortessa 5 laser classical flow cytometer; BD FACSDiscover S8 5-laser spectral imaging cell sorter; BD Rhapsod HT Xpress System; ChemoMetec NucleoCounter NC-3000; Miltenyi gentleMACS Octo dissociator
  • Imaging and Cell Function facility: Olympus FV1000 confocal microscope (Olympus Corp., Japan) system equipped with four lasers, five objectives, and high-sensitivity detectors combined with a flow chamber and injection pump; Applied Biosystems ECIS Ztheta system; Agilent Seahorse XFp analyzer
  • Metabolomics and Proteomics facility: QTRAP 4000 and QTRAP 7500 mass spectrometers; TripleTOF 6600 mass spectrometer connected to HPLC, UHPLC, and nano-HPLC separation systems; Synergy HTX multimode reader, UV-VIS recording spectrophotometer Shimadzu UV 2401 PC; surface plasmon resonance equipment